This study aims to characterise COVID-19 in the paediatric population by studying clinical, serological, biochemical and immunological variables in these patients
Description of the Study:
- Title: Characterization of COVID-19 in the Pediatric Population: Study of Sero-surveillance and of Immunological and Biochemical Biomarkers of Disease Severity.
- Principal Investigators: Iolanda Jordan and Victoria Fumadó.
- Center of Implementation: Hospital Sant Joan de Déu.
- Study Population: Two groups of children with suspected COVID-19 infection (estimate n = 3000): one of them with PCR-confirmed COVID-19 disease and the other with a PCR that is negative for COVID-19. Results obtained from pediatric patients will be compared to a cohort of young adults and pregnant women with PCR-confirmed COVID-19 disease.
- Study Type: Prospective population-based study
- Method: The following samples will be collected:
– Nasopharyngeal-bronchoalveolar aspirate (day 1, day 14, day 40): PCR.
– Saliva and feces samples (day 1): PCR twice a week.
– Capillary blood sample (day 1): Rapid serological test.
– Blood serum and plasma bank (day 1, day 14, day 40): Cytokine quantification and ELISA-based serologic tests.
– Peripheral blood mononuclear cells (PBMCs) (day 1, day 14, day 40-60): Immunophenotyping and antigen specific T-cell activation studies.
– RNA samples: IFN-related transcriptomic analysis (day 1, day 14).
Objectives of the Study:
Principal Objectives: Characterize the SARS-CoV-2 infection in children by:
(1) Studying clinical, serological, biochemical and immunological variables, to identify protective or susceptibility factors determining the clinical spectrum of COVID-19 in this population.
(2) Establishing a hospital-based surveillance system to monitor the evolution of the pandemic in the pediatric population.
(1) Describing the clinical and demographic characteristics of pediatric patients with COVID-19.
(2) Estimating seroprevalences and incidences of SARS-CoV-2 infection in the general and at-risk pediatric populations.
(3) Determining whether there are differences in innate and adaptive immune responses to SARS-CoV-2 depending on patients’ age and/or the disease severity. This specific sub-study of immune responses will compare children with young adults and pregnant women.
(4) Describing the trend of oxidative stress markers and pro-adrenomedullin levels during COVID-19 in pediatric patients, and their changes related to patients’ age and/or the disease severity.
(5) Integrating all immune and biochemical biomarkers, and compare them in terms of their prognostic capacity for poor evolution.
More about this Study:
Sant Joan de Déu: It is a university hospital specializing in the fields of pediatrics, gynecology, and obstetrics, which has a long history of more than 150 years, during which it has undergone many transformations and changes due to the need to adapt both to the social and health conditions of the time and to technological advances and the evolution of health care.
Kids Corona: The Kids Corona platform, which the Sant Joan de Déu Hospital launched last April continues to offer answers to better understand the incidence, impact, and transmission capacity of COVID-19 in children and pregnant women.
Other Kids Corona Projects: “SARS-CoV-2 seroconversion during the postoperative period in pediatric patients“, “Identifying SARS-CoV-2 in breast milk as a potential means of vertical transmission“, “Susceptibility to COVID-19 in pediatric age: Study of cases and household contacts” and “Susceptibility to COVID-19 in Pediatric Age: Study of Cases in School-like Environments”